基于UPLC-Q-TOF MS代谢组学技术筛选表征西门塔尔种公牛精子活力的候选生物标志物

doi:10.11843/j.issn.0366-6964.2019.08.008

Abstract

Abstract: This study aimed to select biomarkers featuring sperm motility in Simmental bull. Twenty four Simmental bulls semen were collected, eleven of which were viewed as abnormal group, and 13 as normal group according to their sperm motility. UPLC-Q-TOF MS metabolomics was used to study change in metabolic profile and metabolites of seminal plasma in the 2 groups. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) revealed that, compared to the normal group, seminal plasma metabolic profiles obviously altered, six metabolites levels significantly decreased(P<0.05), and 19 metabolites levels significantly increased(P<0.05) in abnormal group. These metabolites were further analyzed by ROC curve to observe their discriminating ability to classification. As a result, only 6 metabolites(including sphingosine, N6, N6, N6-Trimethyl-L-lysine, cyclohexylamine, 5'-Deoxyadenosine, pentadecanoic acid and alloxan) had an obviously distinguishing capacity for seminal plasma in the 2 groups. In conclusion, sphingosine, N6, N6, N6-Trimethyl-L-lysine, cyclohexylamine, 5'-Deoxyadenosine, pentadecanoic acid and alloxan in seminal plasma could be better potential biomarkers to evaluate sperm motility in Simmental bull, which could provide new methods and ideas for the study of semen quality determination technology.

CLC Number:

S823.3

LUO Fang, GUO Yansheng, MA Zhiyuan, LU Wangyin, LI Gang, TAO Jinzhong. Selection of Candidate Biomarkers Featuring Sperm Motility in Simmental Bull Based on UPLC-Q-TOF MS Metabolomics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1596-1606.

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Selection of Candidate Biomarkers Featuring Sperm Motility in Simmental Bull Based on UPLC-Q-TOF MS Metabolomics

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